Composite

Part:BBa_K624043:Design

Designed by: Yi-Hong Liou   Group: iGEM11_NYMU-Taipei   (2011-10-05)

RBS + LLO + RBS + Invasin + RBS + ECFP + Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3936
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 770
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1387
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

LLO: PCR amplification from the genomic DNA of Listeria monocytogenes

Inv: PCR amplification from the genomic DNA of Yersinia enterocolitica

References